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pl_violin

Create violin plots to visualize distribution patterns of single-cell RNA sequencing variables, enabling comparison across groups with customizable aesthetics and statistical insights.

Instructions

Plot violin plot of one or more variables.

Input Schema

TableJSON Schema
NameRequiredDescriptionDefault
figsizeNoFigure size. Format is (width, height).
color_mapNoColor map to use for continuous variables.
paletteNoColors to use for plotting categorical annotation groups.
vmaxNoThe value representing the upper limit of the color scale.
vminNoThe value representing the lower limit of the color scale.
vcenterNoThe value representing the center of the color scale.
legend_fontsizeNoNumeric size in pt or string describing the size.
legend_fontweightNoLegend font weight. A numeric value in range 0-1000 or a string.bold
legend_locNoLocation of legend, either 'on data', 'right margin' or a valid keyword for the loc parameter.right margin
legend_fontoutlineNoLine width of the legend font outline in pt.
groupbyNoThe key of the observation grouping to consider.
logNoPlot on logarithmic axis.
use_rawNoUse raw attribute of adata if present.
var_namesNovar_names should be a valid subset of adata.var_names.
layerNoName of the AnnData object layer that wants to be plotted.
gene_symbolsNoColumn name in .var DataFrame that stores gene symbols.
stripplotNoAdd a stripplot on top of the violin plot.
jitterNoAdd jitter to the stripplot (only when stripplot is True).
sizeNoSize of the jitter points.
orderNoOrder in which to show the categories.
scaleNoThe method used to scale the width of each violin.width
keysYesKeys for accessing variables of .var_names or fields of .obs.
multi_panelNoDisplay keys in multiple panels also when groupby is not None.
xlabelNoLabel of the x axis. Defaults to groupby if rotation is None, otherwise, no label is shown.
ylabelNoLabel of the y axis.
rotationNoRotation of xtick labels.

Implementation Reference

  • Handler function that executes the pl_violin tool by dispatching to sc.pl.violin with processed arguments, handles figure saving and logging.
    def run_pl_func(ads, func, arguments): """ Execute a Scanpy plotting function with the given arguments. Parameters ---------- adata : AnnData Annotated data matrix. func : str Name of the plotting function to execute. arguments : dict Arguments to pass to the plotting function. Returns ------- The result of the plotting function. """ adata = ads.adata_dic[ads.active] if func not in pl_func: raise ValueError(f"Unsupported function: {func}") run_func = pl_func[func] parameters = inspect.signature(run_func).parameters kwargs = {k: arguments.get(k) for k in parameters if k in arguments} if "title" not in parameters: kwargs.pop("title", False) kwargs.pop("return_fig", True) kwargs["show"] = False kwargs["save"] = ".png" try: fig = run_func(adata, **kwargs) fig_path = set_fig_path(func, **kwargs) add_op_log(adata, run_func, kwargs) return fig_path except Exception as e: raise e return fig_path
  • Pydantic model defining the input schema and validation for the pl_violin tool.
    class ViolinModel(BaseStatPlotModel): """Input schema for the violin plotting tool.""" keys: Union[str, List[str]] = Field( ..., # Required field description="Keys for accessing variables of .var_names or fields of .obs." ) stripplot: bool = Field( default=True, description="Add a stripplot on top of the violin plot." ) jitter: Union[float, bool] = Field( default=True, description="Add jitter to the stripplot (only when stripplot is True)." ) size: int = Field( default=1, description="Size of the jitter points.", gt=0 ) scale: Literal['area', 'count', 'width'] = Field( default='width', description="The method used to scale the width of each violin." ) order: Optional[List[str]] = Field( default=None, description="Order in which to show the categories." ) multi_panel: Optional[bool] = Field( default=None, description="Display keys in multiple panels also when groupby is not None." ) xlabel: str = Field( default='', description="Label of the x axis. Defaults to groupby if rotation is None, otherwise, no label is shown." ) ylabel: Optional[Union[str, List[str]]] = Field( default=None, description="Label of the y axis." ) rotation: Optional[float] = Field( default=None, description="Rotation of xtick labels." ) @field_validator('size') def validate_size(cls, v: int) -> int: """Validate size is positive""" if v <= 0: raise ValueError("size must be a positive integer") return v
  • Definition of the pl_violin Tool object, including attachment of the input schema.
    pl_violin = types.Tool( name="pl_violin", description="Plot violin plot of one or more variables.", inputSchema=ViolinModel.model_json_schema(), )
  • Mapping of 'pl_violin' to the underlying Scanpy function sc.pl.violin in the pl_func dispatch dictionary.
    "pl_violin": sc.pl.violin,
  • Registration of the pl_violin tool in the pl_tools dictionary, used by server.list_tools().
    "pl_violin": pl_violin,
  • MCP server call_tool handler dispatches pl_violin (and other pl_tools) to run_pl_func.
    elif name in pl_tools.keys(): res = run_pl_func(ads, name, arguments)

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