pl_rank_genes_groups_dotplot
Visualize ranked differentially expressed genes (DEGs) using dot plots to analyze gene expression patterns across groups. Configure color maps, gene lists, and group comparisons for detailed insights in single-cell RNA sequencing studies.
Instructions
Plot ranking of genes(DEGs) using dotplot visualization. Defualt plot DEGs for rank_genes_groups tool
Input Schema
Name | Required | Description | Default |
---|---|---|---|
color_map | No | Color map to use for continuous variables. | |
dendrogram | No | If True or a valid dendrogram key, a dendrogram based on the hierarchical clustering between the groupby categories is added. | |
figsize | No | Figure size. Format is (width, height). | |
gene_symbols | No | Column name in .var DataFrame that stores gene symbols. | |
groupby | Yes | The key of the observation grouping to consider. | |
groups | No | The groups for which to show the gene ranking. | |
key | No | Key used to store the ranking results in adata.uns. | |
layer | No | Name of the AnnData object layer that wants to be plotted. | |
legend_fontoutline | No | Line width of the legend font outline in pt. | |
legend_fontsize | No | Numeric size in pt or string describing the size. | |
legend_fontweight | No | Legend font weight. A numeric value in range 0-1000 or a string. | bold |
legend_loc | No | Location of legend, either 'on data', 'right margin' or a valid keyword for the loc parameter. | right margin |
log | No | Plot on logarithmic axis. | |
min_logfoldchange | No | Value to filter genes in groups if their logfoldchange is less than the min_logfoldchange. | |
n_genes | No | Number of genes to show. This can be a negative number to show down regulated genes. Ignored if var_names is passed. | |
palette | No | Colors to use for plotting categorical annotation groups. | |
use_raw | No | Use raw attribute of adata if present. | |
values_to_plot | No | Instead of the mean gene value, plot the values computed by sc.rank_genes_groups. | |
var_group_labels | No | Labels for each of the var_group_positions that want to be highlighted. | |
var_group_positions | No | Use this parameter to highlight groups of var_names with brackets or color blocks between the given start and end positions. | |
var_names | No | Genes to plot. Sometimes is useful to pass a specific list of var names (e.g. genes) to check their fold changes or p-values | |
vcenter | No | The value representing the center of the color scale. | |
vmax | No | The value representing the upper limit of the color scale. | |
vmin | No | The value representing the lower limit of the color scale. |