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diffmap

Apply diffusion maps to reduce dimensionality in single-cell RNA sequencing data, enabling visualization and analysis of high-dimensional biological datasets.

Instructions

Diffusion Maps for dimensionality reduction

Input Schema

TableJSON Schema
NameRequiredDescriptionDefault
n_compsNoThe number of dimensions of the representation.
neighbors_keyNoIf not specified, diffmap looks .uns['neighbors'] for neighbors settings and .obsp['connectivities'], .obsp['distances'] for connectivities and distances respectively. If specified, diffmap looks .uns[neighbors_key] for neighbors settings and uses the corresponding connectivities and distances.
random_stateNoRandom seed for reproducibility.

Implementation Reference

  • Handler function that executes the tool logic for 'diffmap' by dispatching to sc.tl.diffmap with input arguments, adds operation log, and handles errors.
    def run_tl_func(ads, func, arguments): adata = ads.adata_dic[ads.active] if func not in tl_func: raise ValueError(f"Unsupported function: {func}") run_func = tl_func[func] parameters = inspect.signature(run_func).parameters kwargs = {k: arguments.get(k) for k in parameters if k in arguments} try: res = run_func(adata, **kwargs) add_op_log(adata, run_func, kwargs) except Exception as e: logger.error(f"Error running function {func}: {e}") raise return
  • Pydantic model defining the input schema and validation rules for the 'diffmap' tool.
    class DiffMapModel(JSONParsingModel): """Input schema for the Diffusion Maps dimensionality reduction tool.""" n_comps: int = Field( default=15, description="The number of dimensions of the representation.", gt=0 ) neighbors_key: Optional[str] = Field( default=None, description=( "If not specified, diffmap looks .uns['neighbors'] for neighbors settings " "and .obsp['connectivities'], .obsp['distances'] for connectivities and " "distances respectively. If specified, diffmap looks .uns[neighbors_key] for " "neighbors settings and uses the corresponding connectivities and distances." ) ) random_state: int = Field( default=0, description="Random seed for reproducibility." ) @field_validator('n_comps') def validate_positive_integers(cls, v: int) -> int: """Validate positive integers""" if v <= 0: raise ValueError("n_comps must be a positive integer") return v
  • Registration of the 'diffmap' tool as an MCP Tool object with name, description, and reference to the input schema.
    # Add diffmap tool diffmap_tool = types.Tool( name="diffmap", description="Diffusion Maps for dimensionality reduction", inputSchema=DiffMapModel.model_json_schema(), )
  • Mapping from tool name 'diffmap' to the underlying scanpy function sc.tl.diffmap used by the handler.
    tl_func = { "tsne": sc.tl.tsne, "umap": sc.tl.umap, "draw_graph": sc.tl.draw_graph, "diffmap": sc.tl.diffmap, "embedding_density": sc.tl.embedding_density, "leiden": sc.tl.leiden, "louvain": sc.tl.louvain, "dendrogram": sc.tl.dendrogram, "dpt": sc.tl.dpt, "paga": sc.tl.paga, "ingest": sc.tl.ingest, "rank_genes_groups": sc.tl.rank_genes_groups, "filter_rank_genes_groups": sc.tl.filter_rank_genes_groups, "marker_gene_overlap": sc.tl.marker_gene_overlap, "score_genes": sc.tl.score_genes, "score_genes_cell_cycle": sc.tl.score_genes_cell_cycle, }
  • MCP server list_tools method that includes tl_tools.values(), thereby registering the 'diffmap' tool when MODULE includes 'tl'.
    @server.list_tools() async def list_tools() -> list[types.Tool]: if MODULE == "io": tools = io_tools.values() elif MODULE == "pp": tools = pp_tools.values() elif MODULE == "tl": tools = tl_tools.values() elif MODULE == "pl": tools = pl_tools.values() elif MODULE == "util": tools = util_tools.values() else: tools = [ *io_tools.values(), *pp_tools.values(), *tl_tools.values(), *pl_tools.values(), *util_tools.values(), *ccc_tools.values(), ] return tools

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