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pl_matrixplot

Generate heatmaps to visualize mean expression values across groups using var_names, enabling clear comparison and analysis of single-cell RNA sequencing data with customizable color maps, scales, and grouping options.

Instructions

matrixplot, Create a heatmap of the mean expression values per group of each var_names.

Input Schema

NameRequiredDescriptionDefault
cmapNoString denoting matplotlib color map.viridis
color_mapNoColor map to use for continuous variables.
colorbar_titleNoTitle for the color bar. New line character (\n) can be used.Mean expression in group
dendrogramNoIf True or a valid dendrogram key, a dendrogram based on the hierarchical clustering between the groupby categories is added.
figsizeNoFigure size. Format is (width, height).
gene_symbolsNoColumn name in .var DataFrame that stores gene symbols.
groupbyYesThe key of the observation grouping to consider.
layerNoName of the AnnData object layer that wants to be plotted.
legend_fontoutlineNoLine width of the legend font outline in pt.
legend_fontsizeNoNumeric size in pt or string describing the size.
legend_fontweightNoLegend font weight. A numeric value in range 0-1000 or a string.bold
legend_locNoLocation of legend, either 'on data', 'right margin' or a valid keyword for the loc parameter.right margin
logNoPlot on logarithmic axis.
num_categoriesNoOnly used if groupby observation is not categorical. This value determines the number of groups into which the groupby observation should be subdivided.
paletteNoColors to use for plotting categorical annotation groups.
standard_scaleNoWhether or not to standardize the given dimension between 0 and 1.
swap_axesNoBy default, the x axis contains var_names and the y axis the groupby categories. By setting swap_axes then x are the groupby categories and y the var_names.
use_rawNoUse raw attribute of adata if present.
var_group_labelsNoLabels for each of the var_group_positions that want to be highlighted.
var_group_positionsNoUse this parameter to highlight groups of var_names with brackets or color blocks between the given start and end positions.
var_group_rotationNoLabel rotation degrees. By default, labels larger than 4 characters are rotated 90 degrees.
var_namesNovar_names should be a valid subset of adata.var_names or a mapping where the key is used as label to group the values.
vcenterNoThe value representing the center of the color scale.
vmaxNoThe value representing the upper limit of the color scale.
vminNoThe value representing the lower limit of the color scale.

Input Schema (JSON Schema)

{ "description": "Input schema for the matrixplot plotting tool.", "properties": { "cmap": { "anyOf": [ { "type": "string" }, { "type": "null" } ], "default": "viridis", "description": "String denoting matplotlib color map.", "title": "Cmap" }, "color_map": { "anyOf": [ { "type": "string" }, { "type": "null" } ], "default": null, "description": "Color map to use for continuous variables.", "title": "Color Map" }, "colorbar_title": { "anyOf": [ { "type": "string" }, { "type": "null" } ], "default": "Mean expression\nin group", "description": "Title for the color bar. New line character (\\n) can be used.", "title": "Colorbar Title" }, "dendrogram": { "anyOf": [ { "type": "boolean" }, { "type": "string" } ], "default": false, "description": "If True or a valid dendrogram key, a dendrogram based on the hierarchical clustering between the groupby categories is added.", "title": "Dendrogram" }, "figsize": { "anyOf": [ { "maxItems": 2, "minItems": 2, "prefixItems": [ { "type": "number" }, { "type": "number" } ], "type": "array" }, { "type": "null" } ], "default": null, "description": "Figure size. Format is (width, height).", "title": "Figsize" }, "gene_symbols": { "anyOf": [ { "type": "string" }, { "type": "null" } ], "default": null, "description": "Column name in .var DataFrame that stores gene symbols.", "title": "Gene Symbols" }, "groupby": { "anyOf": [ { "type": "string" }, { "items": { "type": "string" }, "type": "array" } ], "description": "The key of the observation grouping to consider.", "title": "Groupby" }, "layer": { "anyOf": [ { "type": "string" }, { "type": "null" } ], "default": null, "description": "Name of the AnnData object layer that wants to be plotted.", "title": "Layer" }, "legend_fontoutline": { "anyOf": [ { "type": "integer" }, { "type": "null" } ], "default": null, "description": "Line width of the legend font outline in pt.", "title": "Legend Fontoutline" }, "legend_fontsize": { "anyOf": [ { "type": "integer" }, { "type": "number" }, { "type": "string" }, { "type": "null" } ], "default": null, "description": "Numeric size in pt or string describing the size.", "title": "Legend Fontsize" }, "legend_fontweight": { "anyOf": [ { "type": "integer" }, { "type": "string" } ], "default": "bold", "description": "Legend font weight. A numeric value in range 0-1000 or a string.", "title": "Legend Fontweight" }, "legend_loc": { "default": "right margin", "description": "Location of legend, either 'on data', 'right margin' or a valid keyword for the loc parameter.", "title": "Legend Loc", "type": "string" }, "log": { "default": false, "description": "Plot on logarithmic axis.", "title": "Log", "type": "boolean" }, "num_categories": { "default": 7, "description": "Only used if groupby observation is not categorical. This value determines the number of groups into which the groupby observation should be subdivided.", "exclusiveMinimum": 0, "title": "Num Categories", "type": "integer" }, "palette": { "anyOf": [ { "type": "string" }, { "items": { "type": "string" }, "type": "array" }, { "additionalProperties": { "type": "string" }, "type": "object" }, { "type": "null" } ], "default": null, "description": "Colors to use for plotting categorical annotation groups.", "title": "Palette" }, "standard_scale": { "anyOf": [ { "enum": [ "var", "group" ], "type": "string" }, { "type": "null" } ], "default": null, "description": "Whether or not to standardize the given dimension between 0 and 1.", "title": "Standard Scale" }, "swap_axes": { "default": false, "description": "By default, the x axis contains var_names and the y axis the groupby categories. By setting swap_axes then x are the groupby categories and y the var_names.", "title": "Swap Axes", "type": "boolean" }, "use_raw": { "anyOf": [ { "type": "boolean" }, { "type": "null" } ], "default": null, "description": "Use raw attribute of adata if present.", "title": "Use Raw" }, "var_group_labels": { "anyOf": [ { "items": { "type": "string" }, "type": "array" }, { "type": "null" } ], "default": null, "description": "Labels for each of the var_group_positions that want to be highlighted.", "title": "Var Group Labels" }, "var_group_positions": { "anyOf": [ { "items": { "maxItems": 2, "minItems": 2, "prefixItems": [ { "type": "integer" }, { "type": "integer" } ], "type": "array" }, "type": "array" }, { "type": "null" } ], "default": null, "description": "Use this parameter to highlight groups of var_names with brackets or color blocks between the given start and end positions.", "title": "Var Group Positions" }, "var_group_rotation": { "anyOf": [ { "type": "number" }, { "type": "null" } ], "default": null, "description": "Label rotation degrees. By default, labels larger than 4 characters are rotated 90 degrees.", "title": "Var Group Rotation" }, "var_names": { "anyOf": [ { "items": { "type": "string" }, "type": "array" }, { "additionalProperties": { "items": { "type": "string" }, "type": "array" }, "type": "object" } ], "default": null, "description": "var_names should be a valid subset of adata.var_names or a mapping where the key is used as label to group the values.", "title": "Var Names" }, "vcenter": { "anyOf": [ { "type": "string" }, { "type": "number" }, { "items": { "anyOf": [ { "type": "string" }, { "type": "number" } ] }, "type": "array" }, { "type": "null" } ], "default": null, "description": "The value representing the center of the color scale.", "title": "Vcenter" }, "vmax": { "anyOf": [ { "type": "string" }, { "type": "number" }, { "items": { "anyOf": [ { "type": "string" }, { "type": "number" } ] }, "type": "array" }, { "type": "null" } ], "default": null, "description": "The value representing the upper limit of the color scale.", "title": "Vmax" }, "vmin": { "anyOf": [ { "type": "string" }, { "type": "number" }, { "items": { "anyOf": [ { "type": "string" }, { "type": "number" } ] }, "type": "array" }, { "type": "null" } ], "default": null, "description": "The value representing the lower limit of the color scale.", "title": "Vmin" } }, "required": [ "groupby" ], "title": "MatrixplotModel", "type": "object" }

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