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microbe-mcp

下游 微生物组 / 扩增子(16S · ITS) 分析 MCP。把 vegan / DESeq2 / edgeR / igraph / Hmisc / randomForest / ggtree 等 R 脚本封装为工具,由本机 Rscript 产出 png + pdf。

图型对齐两篇公开论文(便于复刻布局,不附带其原始数据):

  • Liu et al. 2023, Nature Microbiology — 稻曲病叶际菌群

  • Zhou et al. 2022, Nature Communications — 番茄枯萎病跨界合成菌群

R 引擎

优先级:MICROBE_RSCRIPT → PATH 上的 RscriptC:\Program Files\R\... / 注册表。

$env:MICROBE_RSCRIPT = "C:\Program Files\R\R-4.5.1\bin\Rscript.exe"
uv run microbe-cli

Related MCP server: methods-mcp

数据契约(3 张 CSV)

文件

必需列

feature_table.csv

feature_id + 各样本计数

taxonomy.csv

feature_id + 界门纲目科属种,或 QIIME 分号串

metadata.csv

sample_name + group;可选环境/代谢物数值列

工具(9)

microbe_env microbe_alpha microbe_beta microbe_composition microbe_diff microbe_network microbe_rf microbe_corr microbe_tree

不含 reads → ASV(DADA2/QIIME2)。已有特征表即可接入。

测试

python tests/prep_test.py   # 合成扩增子表,无真实样本
python tests/smoke.py

注册

{
  "mcpServers": {
    "microbe": {
      "command": "uv",
      "args": ["run", "--directory", "/absolute/path/to/microbe-mcp", "server.py"],
      "env": { "MICROBE_RSCRIPT": "/path/to/Rscript" }
    }
  }
}

许可

MIT。

A
license - permissive license
-
quality - not tested
C
maintenance

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