CRISPR Analysis
crispr_analysisDesign CRISPR guides, score their efficiency, review off-target effects, plan base edits, and estimate repair outcomes for any gene.
Instructions
Merged CRISPR workflow covering guide design, guide scoring, off-target review, base editing, and repair outcome estimation.
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| action | Yes | CRISPR workflow step. | design |
| gene_symbol | No | Target gene symbol. | |
| guide_sequence | No | Guide sequence for scoring, off-target review, or repair analysis. | |
| target_mutation | No | Desired mutation for base-edit design. | |
| repair_template | No | Optional HDR repair template. | |
| cas_variant | No | CRISPR nuclease. | SpCas9 |
| target_region | No | Guide search region, such as 'early_exons' or 'all_coding'. | |
| n_guides | No | Number of guides to return. Default 5. | |
| min_score | No | Minimum guide score for design. | |
| mismatches | No | Maximum mismatches for off-target review. Default 3. | |
| genome | No | Genome assembly used for off-target review. | hg38 |
| cell_line | No | Cell-line context for repair estimates. | |
| use_blast | No | Use BLAST to supplement off-target review. |