design_taqman_probe
Designs a TaqMan probe for the region between two primers, ensuring the probe Tm exceeds primer Tm and follows standard rules.
Instructions
Design a TaqMan probe for the region between two primers. Ensures probe Tm is higher than primers and follows standard rules (no 5' G).
Args: sequence: Target DNA sequence containing the primer binding sites. primer_fwd: Forward primer sequence (5'->3'). primer_rev: Reverse primer sequence (5'->3'). target_probe_tm: Target melting temperature in °C (default: 68.0).
Returns: A JSON string with the designed probe or error message.
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| sequence | Yes | ||
| primer_fwd | Yes | ||
| primer_rev | Yes | ||
| target_probe_tm | No |
Output Schema
| Name | Required | Description | Default |
|---|---|---|---|
| result | Yes |