check_primer_specificity
Check primer specificity by running NCBI BLAST against the genome to identify off-target binding sites.
Instructions
Check if a primer sequence binds elsewhere in the genome using NCBI BLAST. Requires internet connection and may take 10-30 seconds.
Args: primer_sequence: The DNA sequence of the primer (15-30 bp).
Returns: A JSON string with specificity rating and top matches in nt database.
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| primer_sequence | Yes |
Output Schema
| Name | Required | Description | Default |
|---|---|---|---|
| result | Yes |