WetLab-MCP
Server Configuration
Describes the environment variables required to run the server.
| Name | Required | Description | Default |
|---|---|---|---|
No arguments | |||
Instructions
Guidance the server publishes about itself, which clients place ahead of the tool catalog so the model reads it before choosing anything.
This server publishes no instructions, or was last inspected before Glama recorded them.
Capabilities
Features and capabilities supported by this server
Protocol revision2025-11-25
| Capability | Details |
|---|---|
| tools | {
"listChanged": false
} |
| prompts | {
"listChanged": false
} |
| resources | {
"subscribe": false,
"listChanged": false
} |
| experimental | {} |
Tools
Functions exposed to the LLM to take actions
| Name | Description |
|---|---|
| design_qpcr_primersA | Design validated qPCR primer pairs for a given DNA sequence using Primer3. Args: sequence: Target DNA sequence (ATCG only, min 100 bp). target_tm: Target melting temperature in °C (default: 60.0). product_size_range: Desired PCR product size range (e.g., '70-150'). num_pairs: Number of primer pairs to return (1-5, default: 3). Returns: A JSON-formatted string with validated primer pairs or error information. |
| design_cloning_primersA | Design primers for cloning the entire sequence with optional 5' overhangs. Detects common restriction sites and suggests leaders for efficiency. Args: sequence: Target DNA sequence (ORF/Fragment to amplify). fwd_overhang: Optional 5' overhang for forward primer (e.g., restriction site). rev_overhang: Optional 5' overhang for reverse primer. target_tm: Target melting temperature in °C (default: 60.0). Returns: A JSON string with precise and suggested (leader-included) primer pairs. |
| design_taqman_probeA | Design a TaqMan probe for the region between two primers. Ensures probe Tm is higher than primers and follows standard rules (no 5' G). Args: sequence: Target DNA sequence containing the primer binding sites. primer_fwd: Forward primer sequence (5'->3'). primer_rev: Reverse primer sequence (5'->3'). target_probe_tm: Target melting temperature in °C (default: 68.0). Returns: A JSON string with the designed probe or error message. |
| check_primer_specificityA | Check if a primer sequence binds elsewhere in the genome using NCBI BLAST. Requires internet connection and may take 10-30 seconds. Args: primer_sequence: The DNA sequence of the primer (15-30 bp). Returns: A JSON string with specificity rating and top matches in nt database. |
| analyze_multiplex_compatibilityA | Analyze a set of primers for potential cross-reactivity (heterodimers). Useful for multiplex PCR or panel design. Args: primers: A list of primer sequences to analyze. Returns: A JSON string with identified risk interactions and compatibility score. |
| design_multi_gene_panelA | Design a set of non-conflicting primer pairs for multiple genes. Uses a greedy approach to minimize heterodimers between pairs. Args: genes: A list of genes, each with 'name' and 'sequence' keys. target_tm: Target melting temperature for primers in °C (default: 60.0). Returns: A JSON string with the selected non-conflicting panel and any failures. |
Prompts
Interactive templates invoked by user choice
| Name | Description |
|---|---|
No prompts | |
Resources
Contextual data attached and managed by the client
| Name | Description |
|---|---|
No resources | |
TDQS
Scored across 6 tools
Each tool targets a distinct task in primer/probe design: specificity checking, multiplex analysis, cloning primer design, multi-gene panel design, qPCR primer design, and TaqMan probe design. There is no overlap in functionality.
All tool names follow a consistent verb_noun pattern (analyze_, check_, design_), making the tool purpose clear and predictable.
With 6 tools covering the core workflow of primer and probe design and validation, the number is well-suited to the server's purpose without being excessive or insufficient.
The tool set covers the full lifecycle of primer design: specificity checks, compatibility analysis, design for cloning, qPCR, multi-gene panels, and TaqMan probes. No obvious gaps are present for a primer design MCP.