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qc_call_rate

Flag samples and markers failing call-rate thresholds in a variant set, outputting per-sample and per-marker CSV reports with an overall rate summary.

Instructions

Per-sample and per-marker call rate (missingness) QC for a variant set.

Flags samples/markers below the given thresholds. Writes call_rate_samples.csv and call_rate_markers.csv and returns a summary with the overall call rate and the worst offenders. variant_set_db_id is a BrAPI variantSetDbId (from list_content / BrAPI variantsets). For large production sets pass method="allelematrix" with max_markers (e.g. 20000) to estimate from a server-side marker subset instead of a full VCF export. region ("chrom" or "chrom:start-end", 1-based; from list_sequences) restricts the analysis to one genomic window — available on every QC/diversity tool.

Input Schema

TableJSON Schema
NameRequiredDescriptionDefault
methodNoGenotype source: 'vcf' (full export, cached) or 'allelematrix' (paged, server-side subset).vcf
regionNoRestrict analysis to a genomic window: 'chrom' or 'chrom:start-end' (1-based).
output_dirNoDirectory for the output CSV(s) (default ./gigwa_results/<module>/).
max_markersNoCap analysis to the first N markers in canonical Gigwa search order; omit to use all.
variant_set_db_idYesBrAPI variantSetDbId identifying the run (MODULE§project§run) -- copy the exact string from list_variant_sets / list_content, never assemble one by hand: the middle segment is a numeric project index, not the project's name, and a wrong guess fails with an opaque HTTP 500 rather than a clear error.
min_marker_call_rateNoFlag markers with call rate below this (0-1).
min_sample_call_rateNoFlag samples with call rate below this (0-1).

Output Schema

TableJSON Schema
NameRequiredDescriptionDefault
resultYes

Schema Changelog

Changes observed during successful MCP inspections. Dates show when Glama detected each change.

  1. Changed2 schema fields changedv1.9.1
    • changedInput schema / properties / max_markers / description
      Previous value: -"Cap the number of markers analysed (evenly-spaced subsample); omit to use all."New value: +"Cap analysis to the first N markers in canonical Gigwa search order; omit to use all."
    • changedInput schema / properties / variant_set_db_id / description
      Previous value: -"BrAPI variantSetDbId identifying the run (MODULE§project§run); from list_variant_sets / list_content."New value: +"BrAPI variantSetDbId identifying the run (MODULE§project§run) -- copy the exact string from list_variant_sets / list_content, never assemble one by hand: the middle segment is a numeric project index, not the project's name, and a wrong guess fails with an opaque HTTP 500 rather than a clear error."
  2. First observedv1.4.16

TDQS

A4.5/5.0
Behavior4/5

Does the description disclose side effects, auth requirements, rate limits, or destructive behavior?

With no annotations, the description carries the full burden, and it discloses side effects (writes call_rate_samples.csv and call_rate_markers.csv), the return summary, and method behavior (vcf full export vs allelematrix paged subset). It could add detail about output overwrite or directory creation, but the major behavioral traits are transparent.

Agents need to know what a tool does to the world before calling it. Descriptions should go beyond structured annotations to explain consequences.

Conciseness5/5

Is the description appropriately sized, front-loaded, and free of redundancy?

The description is compact and front-loaded: purpose, outputs, and return value come first, followed by targeted parameter guidance. Every sentence carries operational information, with no filler.

Shorter descriptions cost fewer tokens and are easier for agents to parse. Every sentence should earn its place.

Completeness5/5

Given the tool's complexity, does the description cover enough for an agent to succeed on first attempt?

For a 7-parameter QC tool with no annotations and an output schema, the description covers purpose, outputs, return summary, large-set strategy, and cross-tool parameter provenance. The schema covers the remaining details such as defaults and paths, so nothing critical is missing.

Complex tools with many parameters or behaviors need more documentation. Simple tools need less. This dimension scales expectations accordingly.

Parameters4/5

Does the description clarify parameter syntax, constraints, interactions, or defaults beyond what the schema provides?

Schema coverage is 100%, so the baseline is 3, but the description meaningfully adds to several parameters: it explains variant_set_db_id provenance, warns against hand-assembling it, gives the production-scale usage of method and max_markers, and ties region to list_sequences. This exceeds baseline.

Input schemas describe structure but not intent. Descriptions should explain non-obvious parameter relationships and valid value ranges.

Purpose5/5

Does the description clearly state what the tool does and how it differs from similar tools?

The description opens with a specific verb+resource: 'Per-sample and per-marker call rate (missingness) QC for a variant set,' and immediately states what it flags and produces. This clearly distinguishes it from sibling QC tools like qc_heterozygosity and qc_duplicate_accessions.

Agents choose between tools based on descriptions. A clear purpose with a specific verb and resource helps agents select the right tool.

Usage Guidelines4/5

Does the description explain when to use this tool, when not to, or what alternatives exist?

It provides clear context: flags samples/markers below thresholds, writes CSVs, and returns a summary, and it gives concrete guidance for when to use method='allelematrix' with max_markers on large production sets. It does not explicitly name sibling tools as alternatives or state when not to use this tool, so it stops short of a 5.

Agents often have multiple tools that could apply. Explicit usage guidance like "use X instead of Y when Z" prevents misuse.

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