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jean — Molecular Biology & Genomics

verify_read

Read-only

Grade a sequencing read against a reference (✓ verified / ⚠ near / ~ partial / ✗ divergent) from an alignment.

Input Schema

TableJSON Schema
NameRequiredDescriptionDefault
_filesNoThe project: record references shaped like _subject. Their records become library documents a tool names in sequences/parts/document; a trace or alignment is found by file name.
_subjectNoThe record to act on: exactly one of sequence, accession (+source), sample (an id from list_samples) or file ({name, text} or {name, base64}); record_index picks one record of several.
read_nameNowhat to call the read in the report row.read
target_lenYesreference length in bp. In bp.
aligned_refYesgapped reference (target) row.
target_nameNowhat to call the reference in the report row.reference
aligned_readYesgapped read (query) row.
near_coverage_pctNoreference coverage required to grade a read near. In %.
near_ungapped_pctNoungapped identity required to grade a read near — NOT the gap-inclusive `identity` output. In %.
verified_max_gapsNogaps tolerated in a verified alignment.
verified_coverage_pctNoreference coverage required to grade a read verified. In %.
verified_ungapped_pctNoungapped identity required to grade a read verified — NOT the gap-inclusive `identity` output. In %.

Output Schema

TableJSON Schema
NameRequiredDescriptionDefault
reportYesthe row that goes in the record: target, read, grade, ungapped identity, coverage, mismatches and indel events
resultYesthe full alignment result (matches, mismatches, gaps, identities)
statusYesthe grade: verified / near / partial / divergent
identityYesgap-inclusive identity % (BLAST convention)
mismatchesYesmismatch count
coveragePctYeshow much of the reference the read actually covers — a perfect identity over 20% of the target is not a verified clone
indelEventsYesinsertion/deletion EVENTS, not bases — one 12 bp deletion is one event

Schema Changelog

Changes observed during successful MCP inspections.

  1. First observed

TDQS

B3.3/5.0
Behavior3/5

Does the description disclose side effects, auth requirements, rate limits, or destructive behavior?

Annotations already declare readOnlyHint=true, destructiveHint=false, and openWorldHint=false, so the safety profile is fully covered. The description adds the four grade labels and notes the alignment input, but it does not explain threshold behavior or edge cases; those details live mainly in the schema. It adds modest context beyond annotations without contradicting them.

Agents need to know what a tool does to the world before calling it. Descriptions should go beyond structured annotations to explain consequences.

Conciseness5/5

Is the description appropriately sized, front-loaded, and free of redundancy?

The description is a single sentence with no wasted words, and it front-loads the verb, resource, and result taxonomy. Its brevity is appropriate for a tool whose parameters and return value are documented elsewhere.

Shorter descriptions cost fewer tokens and are easier for agents to parse. Every sentence should earn its place.

Completeness3/5

Given the tool's complexity, does the description cover enough for an agent to succeed on first attempt?

An output schema exists, annotations are present, and input-schema coverage is complete, so return values and parameter semantics do not need to be repeated. However, for a 12-parameter tool with nested objects and several sibling alignment/grading tools, the description omits usage context and alternatives, leaving a real but bounded contextual gap.

Complex tools with many parameters or behaviors need more documentation. Simple tools need less. This dimension scales expectations accordingly.

Parameters3/5

Does the description clarify parameter syntax, constraints, interactions, or defaults beyond what the schema provides?

Schema description coverage is 100%, and all 12 parameters, including thresholds and the aligned read/reference rows, are documented directly in the schema. The description adds no parameter-level meaning beyond saying the input is an alignment, so the structured schema is doing the heavy lifting.

Input schemas describe structure but not intent. Descriptions should explain non-obvious parameter relationships and valid value ranges.

Purpose4/5

Does the description clearly state what the tool does and how it differs from similar tools?

States a specific verb (grade), resource (a sequencing read), and comparison basis (against a reference from an alignment), plus the four output grades. It does not distinguish itself from the sibling compare_reads_to_reference or clarify when it is preferred over alignment_agreement, so it falls short of a 5.

Agents choose between tools based on descriptions. A clear purpose with a specific verb and resource helps agents select the right tool.

Usage Guidelines2/5

Does the description explain when to use this tool, when not to, or what alternatives exist?

The description gives no explicit when-to-use guidance, prerequisites, or alternatives. It only implies the operation acts on an alignment, leaving the agent to infer that it belongs after an alignment step and how it differs from sibling grading or comparison tools.

Agents often have multiple tools that could apply. Explicit usage guidance like "use X instead of Y when Z" prevents misuse.

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