miqe_checklist
Read-only
The MIQE 2.0 checklist for a qPCR paper (Bustin et al. 2025, Clin Chem 71:634, Table 1): every item in its five sections, with your answers.
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| _files | No | The project: record references shaped like _subject. Their records become library documents a tool names in sequences/parts/document; a trace or alignment is found by file name. | |
| answers | No | answers by item id, e.g. {"5.8": {"provided": "yes", "description": "Results, Table 2: standard curve over five tenfold dilutions"}, "3.5": {"provided": "no", "description": "DNase-treated RNA validated DNA-free"}}. Ids run section.item over the paper's order (1 Reagent preparation · 2 Sample preparation · 3 Reverse transcription · 4 qPCR protocol · 5 Data analysis). | |
| _subject | No | The record to act on: exactly one of sequence, accession (+source), sample (an id from list_samples) or file ({name, text} or {name, base64}); record_index picks one record of several. |
Output Schema
| Name | Required | Description | Default |
|---|---|---|---|
| tsv | Yes | the checklist as tab-separated text under the paper's own column headers, for a journal submission | |
| rows | Yes | one row per item, in the paper's order: section, id, item, footnotes, provided, description, and the gap footnote b would still send back | |
| notes | Yes | the table's footnotes by letter, which qualify the items that carry them | |
| total | Yes | items in the checklist | |
| reading | Yes | what is answered and what is still owed, generated from the answers | |
| answered | Yes | items answered yes or no | |
| incomplete | Yes | answers without the location (yes) or reason (no) the checklist asks for |