| _files | No | The project: record references shaped like _subject. Their records become library documents a tool names in sequences/parts/document; a trace or alignment is found by file name. | |
| format | No | what to write. Values: csv (CSV order sheet), tsv (TSV), fasta (FASTA), idt (IDT bulk input), samplesheet (Illumina SampleSheet), adapters (Adapter oligos), primers (Tagged primers). | csv |
| prefix | No | name prefix for the rows (BC001, BC002 …). | BC |
| primer | No | the primer the tag is added to. Only applies when format is primers. | |
| _subject | No | The record to act on: exactly one of sequence, accession (+source), sample (an id from list_samples) or file ({name, text} or {name, base64}); record_index picks one record of several. | |
| barcodes | Yes | the index sequences to write out. | |
| adapter_3 | No | sequence 3' of the tag in the adapter — the TruSeq read-1 primer, as the panel offers. Only applies when format is adapters. | ACACTCTTTCCCTACACGACGCTCTTCCGATCT |
| adapter_5 | No | sequence 5' of the tag in the adapter — the Illumina P5, as the panel offers. Only applies when format is adapters. | AATGATACGGCGACCACCGAGATCTACAC |
| idt_scale | No | IDT synthesis scale, exactly as IDT writes it (e.g. 25nm, 100nm, 250nm). Asked for rather than defaulted: which options exist depends on oligo length. Only applies when format is idt. | |
| idt_purification | No | IDT purification (e.g. STD, PAGE, HPLC). Check what your scale allows before ordering. Only applies when format is idt. | |