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Jean — Molecular Biology & Genomics

call_bases_from_trace

Read-only

Call the bases of a Sanger trace from the signal, discarding the instrument's calls and deriving the bases, their positions and their quality from the peaks.

Input Schema

NameRequiredDescriptionDefault
tableNothe instrument/polymer calibration table to score against. The same signal scores differently under each, so a quality value is only interpretable with the table named. Defaults to ABI 3730 POP-7.3730pop7
_filesNoThe project: record references shaped like _subject. Their records become library documents a tool names in sequences/parts/document; a trace or alignment is found by file name.
_subjectNoThe record to act on: exactly one of sequence, accession (+source), sample (an id from list_samples) or file ({name, text} or {name, base64}); record_index picks one record of several.
documentNowhich trace to run on: a file name (`read_F.ab1`) or its project file id. Leave unset to use the trace tab on screen. A file id is project-specific, so a template ships this unset.

Output Schema

NameRequiredDescriptionDefault
basesYeshow many bases the reading called
meanQualityYesthe mean Phred of the reading, on the calibration table it names
instrumentBasesYeshow many the FILE carries, so a gate can see a de-novo reading change the read's length

Schema Changelog

Changes observed during successful MCP inspections.

  1. First observed

TDQS

A3.6/5.0
Behavior3/5

Does the description disclose side effects, auth requirements, rate limits, or destructive behavior?

Annotations already declare readOnlyHint=true and destructiveHint=false, so safety is covered. The description usefully discloses that positions and quality are re-derived from peaks (so results may differ from stored calls), but it does not say whether the derived calls replace the trace's stored calls or only appear in the result, leaving 'discarding' slightly ambiguous against the read-only annotation.

Agents need to know what a tool does to the world before calling it. Descriptions should go beyond structured annotations to explain consequences.

Conciseness5/5

Is the description appropriately sized, front-loaded, and free of redundancy?

One front-loaded sentence that carries the action, the source of truth (signal/peaks) and the outputs (bases, positions, quality) with no filler.

Shorter descriptions cost fewer tokens and are easier for agents to parse. Every sentence should earn its place.

Completeness4/5

Given the tool's complexity, does the description cover enough for an agent to succeed on first attempt?

An output schema exists, so return values need not be described, and all four parameters are fully documented in the schema. What remains missing is only the relationship to sibling basecalling/quality tools, which the description does not cover.

Complex tools with many parameters or behaviors need more documentation. Simple tools need less. This dimension scales expectations accordingly.

Parameters3/5

Does the description clarify parameter syntax, constraints, interactions, or defaults beyond what the schema provides?

Schema description coverage is 100%, and the parameter descriptions already explain the calibration table's effect on quality values, the _subject/_files record selector, and the document/trace target. The description adds no parameter-level meaning beyond the schema, so the baseline 3 applies.

Input schemas describe structure but not intent. Descriptions should explain non-obvious parameter relationships and valid value ranges.

Purpose4/5

Does the description clearly state what the tool does and how it differs from similar tools?

States a specific verb and resource (call the bases of a Sanger trace) plus the mechanism (derive from the signal/peaks). The phrase 'discarding the instrument's calls' implicitly distinguishes it from adopt_trace_calls and restore_trace_file_calls, but it never names those siblings explicitly.

Agents choose between tools based on descriptions. A clear purpose with a specific verb and resource helps agents select the right tool.

Usage Guidelines3/5

Does the description explain when to use this tool, when not to, or what alternatives exist?

Usage is implied rather than stated: you use this when you want bases re-derived from the raw peaks instead of trusting the instrument's basecaller. There is no explicit when-not guidance and no routing to near neighbours such as call_mixed_bases_from_trace or recompute_trace_quality.

Agents often have multiple tools that could apply. Explicit usage guidance like "use X instead of Y when Z" prevents misuse.

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