Sanger vs Reference
sanger_vs_referenceAlign a Sanger ABIF read to a reference and report identity plus every mismatch, insertion and deletion.
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| read | No | Sanger read as FASTA or raw text (alternative to uploading an ABIF trace). Also the per-record field for plate-batch runs via /api/v1/batch. | |
| fileName | No | Optional original file name (echoed back). | |
| reference | Yes | Expected reference sequence (FASTA or raw). | |
| fileBase64 | No | The binary ABIF (.ab1 / .abi) trace file, base64-encoded. | |
| minCoverage | No | Fraction of the reference the read must span before a PASS is meaningful; below this the verdict is 'ambiguous_low_coverage' regardless of identity. Lower it when the reference is intentionally just the region/junction being checked. |