Primer Tm calculator
melting_temperaturePrimer/oligo melting temperature: nearest-neighbour (SantaLucia 1998) at the supplied reaction conditions, recommended from 14 nt up, with the Wallace rule for shorter oligos, a fixed-100 mM-Na+ Schildkraut-Lifson reference estimate, and molecular weights.
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| mgMM | No | Divalent cation [Mg2+] (mM). | |
| naMM | No | Monovalent cation [Na+]/[K+] (mM). | |
| dntpMM | No | Total [dNTP] (mM), chelates Mg2+. | |
| oligoNM | No | Total strand concentration (nM). | |
| sequence | Yes | Nucleotide sequence (raw or FASTA; IUPAC accepted). | |
| targetTm | No | Optional target Tm (°C). When given, the response includes a pass/fail gate. | |
| tmTolerance | No | Allowed +/- window (°C) around targetTm for the gate. |