trace_raw_signal
The UNPROCESSED signal of a Sanger trace: how many scans the instrument recorded, where each channel's baseline sits, the peak raw value, and how many scans analysis discarded off the front as dye front.
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| _files | No | The project: record references shaped like _subject. Their records become library documents a tool names in sequences/parts/document; a trace or alignment is found by file name. | |
| _subject | No | The record to act on: exactly one of sequence, accession (+source), sample (an id from list_samples) or file ({name, text} or {name, base64}); record_index picks one record of several. | |
| document | No | which trace file to read: its name (`read_F.ab1`) or its project file id. A file id is project-specific, so a template ships this unset. |
Output Schema
| Name | Required | Description | Default |
|---|---|---|---|
| baseline | Yes | per-channel median raw sample (A/C/G/T) — the offset analysis subtracts | |
| rawScans | Yes | scans the instrument recorded, on its own time base | |
| peakRawValue | Yes | the largest raw sample across all four channels | |
| baselineSpread | Yes | highest channel baseline minus lowest. A large spread is one dye channel sitting high, which analysis removes and a raw view shows | |
| droppedFromFront | Yes | scans analysis discarded off the front (dye front and primer peak). Gate on it: an unusually long discard is a run that took a long time to resolve |