ligation_independent_clone
Join fragments without a ligase by one of the five named methods — LIC, SLIC, In-Fusion, CPEC or USER (pydna).
Input Schema
| Name | Required | Description | Default |
|---|---|---|---|
| _files | No | The project: record references shaped like _subject. Their records become library documents a tool names in sequences/parts/document; a trace or alignment is found by file name. | |
| arm_nt | No | homology per junction. Left unset, the method's own recommended arm is used (In-Fusion widens its own for more than one insert). In nt. | |
| method | No | the cloning method, which decides the chemistry: LIC and SLIC and In-Fusion chew 3' ends, CPEC removes nothing, USER excises a uracil. Values: lic (LIC), slic (SLIC), in-fusion (In-Fusion), cpec (CPEC), user (USER). | in-fusion |
| _subject | No | The record to act on: exactly one of sequence, accession (+source), sample (an id from list_samples) or file ({name, text} or {name, base64}); record_index picks one record of several. | |
| fragments | No | the fragments to join — wire upstream `record` outputs here, or name library documents. | |
| circular_only | No | report only circular products. | |
| include_subject | No | also include the node's subject as a fragment. |
Output Schema
| Name | Required | Description | Default |
|---|---|---|---|
| arm | Yes | the homology arm the run used | |
| record | Yes | the first product, as a record | |
| summary | Yes | what the reaction gave, naming the method and its chemistry | |
| products | Yes | every product | |
| armVerdict | Yes | whether that arm is one the method's authority stands behind, in its own words | |
| productCount | Yes | number of products (>1 = ambiguous) |