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fusion_pcr

Overlap-extension (fusion) PCR — join fragments that share terminal homology into one product, carrying their features across (pydna). Emits the fused product as a NEW record.

Input Schema

NameRequiredDescriptionDefault
limitNominimum shared terminal homology two fragments must have to fuse (pydna's own default is 15). In bp.
_filesNoThe project: record references shaped like _subject. Their records become library documents a tool names in sequences/parts/document; a trace or alignment is found by file name.
_subjectNoThe record to act on: exactly one of sequence, accession (+source), sample (an id from list_samples) or file ({name, text} or {name, base64}); record_index picks one record of several.
fragmentsNothe fragments to fuse, in order — wire upstream `record` outputs here, or name library documents.
include_subjectNoalso include the node's subject as the first fragment.

Output Schema

NameRequiredDescriptionDefault
recordYesthe fused product, as a record
reportYesthe engine's own summary (count + sizes)
productsYesevery fused product
junctionsYesthe first product's joins: each overlap's product coordinates (0-based inclusive), bases, length and Tm
productTmYesproduct melting temperature of the first product
productCountYesnumber of products (>1 = ambiguous fusion)

Schema Changelog

Changes observed during successful MCP inspections.

  1. Changed1 schema field changed
    • addedOutput schema / properties / productTm / x-unit
      Added value: +"°C"
  2. First observed

TDQS

A3.8/5.0
Behavior4/5

Does the description disclose side effects, auth requirements, rate limits, or destructive behavior?

Annotations already mark it as non-read-only and non-destructive. The description adds real value beyond that: it discloses the engine (pydna) and, importantly, that output is a NEW record rather than in-place modification. It doesn't mention failure behavior when homology is insufficient or the limit constraint, so not a 5.

Agents need to know what a tool does to the world before calling it. Descriptions should go beyond structured annotations to explain consequences.

Conciseness5/5

Is the description appropriately sized, front-loaded, and free of redundancy?

Two tight sentences, front-loaded with the mechanism and ending with the output behavior. No filler, every clause earns its place.

Shorter descriptions cost fewer tokens and are easier for agents to parse. Every sentence should earn its place.

Completeness4/5

Given the tool's complexity, does the description cover enough for an agent to succeed on first attempt?

With annotations and an output schema present, the description needn't explain returns, and the 'NEW record' note covers the key behavioral question. The main gap is absence of routing guidance to sibling assembly tools, but for correct invocation the definition is otherwise sufficient.

Complex tools with many parameters or behaviors need more documentation. Simple tools need less. This dimension scales expectations accordingly.

Parameters3/5

Does the description clarify parameter syntax, constraints, interactions, or defaults beyond what the schema provides?

Schema description coverage is 100%, so baseline is 3. The description reinforces that fragments are fused 'in order' and that features carry across, but the limit/homology and include_subject meanings are already fully documented in the schema, so little is added beyond it.

Input schemas describe structure but not intent. Descriptions should explain non-obvious parameter relationships and valid value ranges.

Purpose4/5

Does the description clearly state what the tool does and how it differs from similar tools?

States a specific mechanism (overlap-extension/fusion PCR) and resource: joins fragments sharing terminal homology into one product, carrying features across, via pydna. An agent can identify the operation clearly. It doesn't explicitly contrast with adjacent joining siblings like gibson_assemble or ligate_fragments, so it falls short of a 5.

Agents choose between tools based on descriptions. A clear purpose with a specific verb and resource helps agents select the right tool.

Usage Guidelines3/5

Does the description explain when to use this tool, when not to, or what alternatives exist?

Usage is implied by the homology requirement ('fragments that share terminal homology'), which is a useful selection condition. However, there is no explicit when-to-use, when-not, or named alternative among the many assembly tools (gibson_assemble, ligate_fragments, golden_gate_assemble), leaving routing to inference.

Agents often have multiple tools that could apply. Explicit usage guidance like "use X instead of Y when Z" prevents misuse.

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