Consolidated Multi-Attribute Method hotspot report: every sequence-liable modification site in one ranked list, across seven modification types — deamidation, oxidation, isomerization, N-glycosylation sequons, acid-labile cleavage, N-terminal pyroglutamate, and C-terminal lysine clipping. This is the site list a MAM peptide-mapping method has to cover, and the starting point for an attribute-criticality assessment.

Sequence-derived only: it tells you which residues CAN carry an attribute, not which ones DO in a given lot. It replaces neither the LC-MS measurement nor the criticality ranking.

Parameters:
  sequence: Protein/antibody amino acid sequence.

Returns: {hotspots: [{position, modification_type, motif, risk, context}]}